This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fisher, S. H.
Right arrow Articles by Wray, L. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fisher, S. H.
Right arrow Articles by Wray, L. V., Jr.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2002, p. 2148-2154, Vol. 184, No. 8
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.8.2148-2154.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Bacillus subtilis 168 Contains Two Differentially Regulated Genes Encoding L-Asparaginase

Susan H. Fisher* and Lewis V. Wray, Jr.

Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts 02118

Received 27 November 2001/ Accepted 18 January 2002

Expression of the two Bacillus subtilis genes encoding L-asparaginase is controlled by independent regulatory factors. The ansZ gene (formerly yccC) was shown by mutational analysis to encode a functional L-asparaginase, the expression of which is activated during nitrogen-limited growth by the TnrA transcription factor. Gel mobility shift and DNase I footprinting experiments indicate that TnrA regulates ansZ expression by binding to a DNA site located upstream of the ansZ promoter. The expression of the ansA gene, which encodes the second L-asparaginase, was found to be induced by asparagine. The ansA repressor, AnsR, was shown to negatively regulate its own expression.

* Corresponding author. Mailing address: Department of Microbiology, Boston University School of Medicine, 715 Albany St., Boston, MA 02118. Phone: (617) 638-5498. Fax: (617) 638-4286. E-mail: shfisher{at}bu.edu.

Journal of Bacteriology, April 2002, p. 2148-2154, Vol. 184, No. 8
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.8.2148-2154.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Fisher, S. H., Wray, L. V. Jr. (2009). Novel trans-Acting Bacillus subtilis glnA Mutations That Derepress glnRA Expression. J. Bacteriol. 191: 2485-2492 [Abstract] [Full Text]  
  • Fisher, S. H., Wray, L. V. Jr. (2008). Bacillus subtilis glutamine synthetase regulates its own synthesis by acting as a chaperone to stabilize GlnR-DNA complexes. Proc. Natl. Acad. Sci. USA 105: 1014-1019 [Abstract] [Full Text]  
  • Thomaides, H. B., Davison, E. J., Burston, L., Johnson, H., Brown, D. R., Hunt, A. C., Errington, J., Czaplewski, L. (2007). Essential Bacterial Functions Encoded by Gene Pairs. J. Bacteriol. 189: 591-602 [Abstract] [Full Text]  
  • Wray, L. V. Jr., Fisher, S. H. (2007). Functional Analysis of the Carboxy-Terminal Region of Bacillus subtilis TnrA, a MerR Family Protein. J. Bacteriol. 189: 20-27 [Abstract] [Full Text]  
  • Fisher, S. H., Wray, L. V. Jr (2006). Feedback-Resistant Mutations in Bacillus subtilis Glutamine Synthetase Are Clustered in the Active Site.. J. Bacteriol. 188: 5966-5974 [Abstract] [Full Text]  
  • Zalieckas, J. M., Wray, L. V. Jr., Fisher, S. H. (2006). Cross-Regulation of the Bacillus subtilis glnRA and tnrA Genes Provides Evidence for DNA Binding Site Discrimination by GlnR and TnrA.. J. Bacteriol. 188: 2578-2585 [Abstract] [Full Text]  
  • Tojo, S., Satomura, T., Morisaki, K., Yoshida, K.-I., Hirooka, K., Fujita, Y. (2004). Negative Transcriptional Regulation of the ilv-leu Operon for Biosynthesis of Branched-Chain Amino Acids through the Bacillus subtilis Global Regulator TnrA. J. Bacteriol. 186: 7971-7979 [Abstract] [Full Text]  
  • Belitsky, B. R., Sonenshein, A. L. (2004). Modulation of Activity of Bacillus subtilis Regulatory Proteins GltC and TnrA by Glutamate Dehydrogenase. J. Bacteriol. 186: 3399-3407 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»