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Journal of Bacteriology, May 2002, p. 2481-2490, Vol. 184, No. 9
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.9.2481-2490.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

nblS, a Gene Involved in Controlling Photosynthesis-Related Gene Expression during High Light and Nutrient Stress inSynechococcus elongatus PCC 7942

Lorraine G. van Waasbergen,1* Nadia Dolganov,2 and Arthur R. Grossman3

Department of Biology, University of Texas at Arlington, Arlington, Texas 76019,1 Department of Microbiology and Immunology, Stanford Medical School, Stanford University,2 Department of Plant Biology, The Carnegie Institution of Washington, Stanford, California 943053

Received 29 October 2001/ Accepted 3 February 2002

The HliA protein of the cyanobacterium Synechococcus elongatus PCC 7942 is a small, thylakoid-associated protein that appears to play a role in photoprotection; its transcript rapidly accumulates in response to high-intensity light (HL) and the hli gene family is required for survival of cells in high light. In order to discover regulatory factors involved in HL acclimation in cyanobacteria, a screen was performed for chemically generated mutants unable to properly control expression of the hliA gene in response to HL. One such mutant was identified, and complementation analysis led to the identification of the affected gene, designated nblS. Based on its deduced protein sequence, NblS appears to be a membrane-bound, PAS-domain-bearing, sensor histidine kinase of two-component regulatory systems in bacteria. The nblS mutant was unable to properly control light intensity-mediated expression of several other photosynthesis-related genes, including all three psbA genes and the cpcBA genes. The mutant was also unable to control expression of the hliA and psbA genes in response to low-intensity blue/UV-A light, a response that may be related to the HL-mediated regulation of the genes. Additionally, in response to nutrient deprivation, the nblS mutant was unable to properly control accumulation of the nblA transcript and associated degradation of the light-harvesting phycobilisomes. The nblS mutant dies more rapidly than wild-type cells following exposure to HL or nutrient deprivation, likely due to its inability to properly acclimate to these stress conditions. Thus, the NblS protein is involved in the control of a number of processes critical for altering the photosynthetic apparatus in response to both HL and nutrient stress conditions.

* Corresponding author. Mailing address: Department of Biology, Box 19498, University of Texas at Arlington, Arlington, TX 76019. Phone: (817) 272-5239. FAX: (817) 272-2855. E-mail: Lorivw{at}uta.edu.

Journal of Bacteriology, May 2002, p. 2481-2490, Vol. 184, No. 9
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.9.2481-2490.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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