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Journal of Bacteriology, May 2002, p. 2533-2538, Vol. 184, No. 9
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.9.2533-2538.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genetic Organization of the Vibrio harveyi dnaA Gene Region and Analysis of the Function of the V. harveyi DnaA Protein in Escherichia coli

Dvora Berenstein,1 Kirsten Olesen,1 Christian Speck,2,3 and Ole Skovgaard1*

Department of Life Sciences and Chemistry, Roskilde University, DK-4000 Roskilde, Denmark,1 Max-Planck-Institut für Molekulare Genetik, D-14195 Berlin-Dahlem, Germany,2 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 117243

Received 9 October 2001/ Accepted 7 February 2002

The Vibrionaceae family is distantly related to Enterobacteriaceae within the group of bacteria possessing the Dam methylase system. We have cloned, sequenced, and analyzed the dnaA gene region of Vibrio harveyi and found that although the organization of the V. harveyi dnaA region differs from that of Escherichia coli, the expression of both genes is autoregulated and ATP-DnaA binds cooperatively to ATP-DnaA boxes in the dnaA promoter region. The DnaA proteins of V. harveyi and E. coli are interchangeable and function nearly identically in controlling dnaA transcription and the initiation of chromosomal DNA replication despite the evolutionary distance between these bacteria.

* Corresponding author. Mailing address: Department of Life Sciences and Chemistry, 17-2, Roskilde University, P.O. Box 260, DK-4000 Roskilde, Denmark. Phone: 45 4674 2405. Fax: 45 4674 3011. E-mail: olesk{at}ruc.dk.

Journal of Bacteriology, May 2002, p. 2533-2538, Vol. 184, No. 9
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.9.2533-2538.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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  • Grigorian, A. V., Lustig, R. B., Guzman, E. C., Mahaffy, J. M., Zyskind, J. W. (2003). Escherichia coli Cells with Increased Levels of DnaA and Deficient in Recombinational Repair Have Decreased Viability. J. Bacteriol. 185: 630-644 [Abstract] [Full Text]  


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