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Journal of Bacteriology, May 2002, p. 2557-2560, Vol. 184, No. 9
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.9.2557-2560.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Nitrite Reductase of Nitrosomonas europaea Is Not Essential for Production of Gaseous Nitrogen Oxides and Confers Tolerance to Nitrite

Hubertus J. E. Beaumont,1 Norman G. Hommes,2 Luis A. Sayavedra-Soto,2 Daniel J. Arp,2 David M. Arciero,3 Alan B. Hooper,3 Hans V. Westerhoff,1 and Rob J. M. van Spanning1*

BioCentrum Amsterdam, Department of Molecular Cell Physiology, Vrije Universiteit, NL-1081 Amsterdam, The Netherlands,1 Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331-2902,2 Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, St. Paul, Minnesota 551083

Received 27 November 2001/ Accepted 1 February 2002

A gene that encodes a periplasmic copper-type nitrite reductase (NirK) was identified in Nitrosomonas europaea. Disruption of this gene resulted in the disappearance of Nir activity in cell extracts. The nitrite tolerance of NirK-deficient cells was lower than that of wild-type cells. Unexpectedly, NirK-deficient cells still produced nitric oxide (NO) and nitrous oxide (N2O), the latter in greater amounts than that of wild-type cells. This demonstrates that NirK is not essential for the production of NO and N2O by N. europaea. Inactivation of the putative fnr gene showed that Fnr is not essential for the expression of nirK.

* Corresponding author. Mailing address: Department of Molecular Cell Physiology, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV Amsterdam, The Netherlands. Phone: 31-20-4447179. Fax: 31-20-4447229. E-mail: spanning{at}bio.vu.nl.

Journal of Bacteriology, May 2002, p. 2557-2560, Vol. 184, No. 9
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.9.2557-2560.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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