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Journal of Bacteriology, May 2003, p. 3210-3213, Vol. 185, No. 10
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.10.3210-3213.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Characterization of the Chlorate Reductase from Pseudomonas chloritidismutans

Arthur F. W. M. Wolterink,1 Emile Schiltz,2 Peter-Leon Hagedoorn,3 Wilfred R. Hagen,3 Servé W. M. Kengen,1 and Alfons J. M. Stams1*

Laboratory of Microbiology, Wageningen University, 6703 CT Wageningen,1 Kluyver Department of Biotechnology, University of Delft, 2628 BC Delft, The Netherlands,3 Institute for Organic Chemistry and Biochemistry, University of Freiburg, D-79104 Freiburg, Germany2

Received 23 December 2002/ Accepted 26 February 2003

A chlorate reductase has been purified from the chlorate-reducing strain Pseudomonas chloritidismutans. Comparison with the periplasmic (per)chlorate reductase of strain GR-1 showed that the cytoplasmic chlorate reductase of P. chloritidismutans reduced only chlorate and bromate. Differences were also found in N-terminal sequences, molecular weight, and subunit composition. Metal analysis and electron paramagnetic resonance measurements showed the presence of iron and molybdenum, which are also found in other dissimilatory oxyanion reductases.

* Corresponding author. Mailing address: Laboratory of Microbiology, Wageningen University, H. van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands, Phone: 31 317 483101. Fax: 31 317 483829. E-mail: fons.stams{at}wur.nl.

Journal of Bacteriology, May 2003, p. 3210-3213, Vol. 185, No. 10
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.10.3210-3213.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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