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Journal of Bacteriology, May 2003, p. 3223-3227, Vol. 185, No. 10
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.10.3223-3227.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Single Superoxide Dismutase of Rhodobacter capsulatus Is a Cambialistic, Manganese-Containing Enzyme

Leandro C. Tabares, Cristian Bittel, Néstor Carrillo, Ana Bortolotti, and Néstor Cortez*

Instituto de Biología Molecular y Celular de Rosario, Universidad Nacional de Rosario and CONICET, S2002LRK Rosario, Argentina

Received 9 December 2002/ Accepted 6 March 2003

The phototrophic bacterium Rhodobacter capsulatus contains a single, oxygen-responsive superoxide dismutase (SODRc) homologous to iron-containing superoxide dismutase enzymes. Recombinant SODRc, however, displayed higher activity after refolding with Mn2+, especially when the pH of the assay mixture was raised. SODRc isolated from Rhodobacter cells also preferentially contains manganese, but metal discrimination depends on the culture conditions, with iron fractions increasing from 7% in aerobic cultures up to 40% in photosynthetic cultures. Therefore, SODRc behaves as a Mn-containing dismutase with cambialistic properties.

* Corresponding author. Mailing address: IBR, Área Biología Molecular, Facultad de Cs. Bioquímicas y Farmacéuticas, U.N.R., Suipacha 531, S2002LRK Rosario, Argentina. Phone: (54) (341) 435 0661/0596. Fax: (54) (341) 439 0465. E-mail: cortez{at}arnet.com.ar.

Journal of Bacteriology, May 2003, p. 3223-3227, Vol. 185, No. 10
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.10.3223-3227.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kim, E.-J., Kim, J.-S., Lee, I.-H., Rhee, H. J., Lee, J. K. (2008). Superoxide Generation by Chlorophyllide a Reductase of Rhodobacter sphaeroides. J. Biol. Chem. 283: 3718-3730 [Abstract] [Full Text]  
  • Wintjens, R., Noel, C., May, A. C. W., Gerbod, D., Dufernez, F., Capron, M., Viscogliosi, E., Rooman, M. (2004). Specificity and Phenetic Relationships of Iron- and Manganese-containing Superoxide Dismutases on the Basis of Structure and Sequence Comparisons. J. Biol. Chem. 279: 9248-9254 [Abstract] [Full Text]  


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