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Journal of Bacteriology, May 2003, p. 3238-3243, Vol. 185, No. 10
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.10.3238-3243.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The yggH Gene of Escherichia coli Encodes a tRNA (m7G46) Methyltransferase

Lara G. S. De Bie,1 Martine Roovers,1 Yamina Oudjama,2 Ruddy Wattiez,3 Catherine Tricot,2 Victor Stalon,1,2 Louis Droogmans,1* and Janusz M. Bujnicki4

Laboratoire de Microbiologie, Université Libre de Bruxelles,1 Institut de Recherches Microbiologiques Jean-Marie Wiame, B-1070 Brussels,2 Laboratoire de Chimie Biologique, Université de Mons-Hainaut, B-7000 Mons, Belgium,3 Bioinformatics Laboratory, International Institute of Molecular and Cell Biology, 02-109 Warsaw, Poland4

Received 20 September 2002/ Accepted 3 March 2003

We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-L-methionine-dependent formation of N7-methylguanosine at position 46 (m7G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m7G46) methyltransferase activity.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, Université Libre de Bruxelles, Institut de Recherche Microbiologiques J.-M. Wiame, 1, avenue E. Gryson, B-1070 Brussels, Belgium. Phone: 32 2 526 7254. Fax: 32 2 526 7273. E-mail: louisd{at}dbm.ulb.ac.be.

Journal of Bacteriology, May 2003, p. 3238-3243, Vol. 185, No. 10
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.10.3238-3243.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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