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Journal of Bacteriology, June 2003, p. 3453-3457, Vol. 185, No. 11
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.11.3453-3457.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Conserved Eukaryotic Histone-Fold Residues Substituted into an Archaeal Histone Increase DNA Affinity but Reduce Complex Flexibility

Divya J. Soares,{dagger} Frédéric Marc,{ddagger} and John N. Reeve*

Department of Microbiology, The Ohio State University, Columbus, Ohio 43210

Received 14 January 2003/ Accepted 21 March 2003

Although the archaeal and eukaryotic nucleosome core histones evolved from a common ancestor, conserved lysine residues are present at DNA-binding locations in all four eukaryotic histones that are not present in the archaeal histones. Introduction of lysine residues at the corresponding locations into an archaeal histone, HMfB, generated a variant with increased affinity for DNA that formed more compact complexes with DNA. However, these complexes no longer facilitated the circularization of short DNA molecules and had lost the flexibility to wrap DNA alternatively in either a negative or positive supercoil.

* Corresponding author. Mailing address: Department of Microbiology, The Ohio State University, 484 W. 12th Ave., Columbus, OH 43210-1292. Phone: (614) 292-2301. Fax: (614) 292-8120. E-mail: reeve.2{at}osu.edu.

{dagger} Present address: Chemical Abstracts, Columbus, OH 43210.

{ddagger} Present address: ProtNeteomix, 44000, Nantes, France.

Journal of Bacteriology, June 2003, p. 3453-3457, Vol. 185, No. 11
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.11.3453-3457.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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