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Journal of Bacteriology, June 2003, p. 3476-3479, Vol. 185, No. 11
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.11.3476-3479.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Glycosyltransferase Gene Encoding the Enzyme Catalyzing the First Step of Mycothiol Biosynthesis (mshA)

Gerald L. Newton,1 Teresa Koledin,1 Batia Gorovitz,2 Mamta Rawat,2 Robert C. Fahey,1* and Yossef Av-Gay2

Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093,1 Department of Medicine, Division of Infectious Diseases, University of British Columbia, Vancouver, British Columbia V5Z 3J5, Canada2

Received 18 October 2002/ Accepted 6 March 2003

Mycothiol is the major thiol present in most actinomycetes and is produced from the pseudodisaccharide 1D-myo-inosityl 2-acetamido-2-deoxy-{alpha}-D-glucopyranoside (GlcNAc-Ins). A transposon mutant of Mycobacterium smegmatis shown to be GlcNAc-Ins and mycothiol deficient was sequenced to identify a putative glycosyltransferase gene designated mshA. The ortholog in Mycobacterium tuberculosis, Rv0486, was used to complement the mutant phenotype.

* Corresponding author. Mailing address: Depatment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093-0506. Phone: (619) 534-2163. Fax: (619) 534-4864. E-mail: rcfahey{at}ucsd.edu.

Journal of Bacteriology, June 2003, p. 3476-3479, Vol. 185, No. 11
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.11.3476-3479.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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