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Journal of Bacteriology, June 2003, p. 3558-3566, Vol. 185, No. 12
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.12.3558-3566.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Posttranscriptional Control of Quorum-Sensing-Dependent Virulence Genes by DksA in Pseudomonas aeruginosa

Florence Jude,1,2 Thilo Köhler,1 Pavel Branny,1,3 Karl Perron,1 Matthias P. Mayer,4 Rachel Comte,1 and Christian van Delden1*

Department of Genetics and Microbiology, Centre Médical Universitaire, University of Geneva, Geneva, Switzerland,1 Laboratoire d'Océanographie Biologique, Université Bordeaux I, Bordeaux, France,2 Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic,3 Zentrum für Molekulare Biologie, Universität Heidelberg, Heidelberg, Germany4

Received 7 October 2002/ Accepted 26 March 2003

Pseudomonas aeruginosa controls the secretion of extracellular virulence factors, including rhamnolipids and LasB elastase, by the las and rhl quorum-sensing systems. Here, we mutated the dksA gene of P. aeruginosa by insertion of an {Omega}-Hg cassette. The mutant displayed growth rates similar to that of the wild type in rich medium but was impaired in growth in defined minimal medium. Production of rhamnolipids and LasB elastase by the dksA mutant was only 4 and 10%, respectively, of wild-type levels. These defects could be partially complemented by introduction of the plasmid-encoded dksA genes from P. aeruginosa or Escherichia coli. In the dksA mutant, the expression of rhlI was increased early during exponential growth, but expression of other quorum-sensing regulator genes—lasR, lasI, and rhlR—was not affected. Although the transcription of the lasB and rhlAB genes was comparable between the dksA mutant and the wild-type strain in peptone tryptic soy broth medium, we observed reduced translation of both genes in the dksA mutant. Similarly, we found that full translation of lasB and rhlAB genes in E. coli also requires the dksA gene. DksA is therefore a novel regulator involved in the posttranscriptional control of extracellular virulence factor production in P. aeruginosa.

* Corresponding author. Mailing address: Department of Genetics and Microbiology, CMU, 9, av. de Champel, CH-1211 Geneva 4, Switzerland. Phone: 41-22-7025639. Fax: 41-22-7025702. E-mail: Christian.vandelden{at}medecine.unige.ch.

Journal of Bacteriology, June 2003, p. 3558-3566, Vol. 185, No. 12
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.12.3558-3566.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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