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Journal of Bacteriology, July 2003, p. 3753-3763, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3753-3763.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of Transposable Elements of Paracoccus pantotrophus

Dariusz Bartosik,^* Marta Sochacka, and Jadwiga Baj

Warsaw University, Institute of Microbiology, Department of Bacterial Genetics, 02-096 Warsaw, Poland

Received 10 March 2003/ Accepted 18 April 2003

We studied diversity and distribution of transposable elements residing in different strains (DSM 11072, DSM 11073, DSM 65, and LMD 82.5) of a soil bacterium Paracoccus pantotrophus (-Proteobacteria). With application of a shuttle entrapment vector pMEC1, several novel insertion sequences (ISs) and transposons (Tns) have been identified. They were sequenced and subjected to detailed comparative analysis, which allowed their characterization (i.e., identification of transposase genes, terminal inverted repeats, as well as target sequences) and classification into the appropriate IS or Tn families. The frequency of transposition of these elements varied and ranged from 10^-6 to 10^-3 depending on the strain. The copy number, localization (plasmid or chromosome), and distribution of these elements in the Paracoccus species P. pantotrophus, P. denitrificans, P. methylutens, P. solventivorans, and P. versutus were analyzed. This allowed us to distinguish elements that are common in paracocci (ISPpa2, ISPpa3—both of the IS5 family—and ISPpa5 of IS66 family) as well as strain-specific ones (ISPpa1 of the IS256 family, ISPpa4 of the IS5 family, and Tn3434 and Tn5393 of the Tn3 family), acquired by lateral transfer events. These elements will be of a great value in the design of new genetic tools for paracocci, since only one element (IS1248 of P. denitrificans) has been described so far in this genus.

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* Corresponding author. Mailing address: Warsaw University, Institute of Microbiology, Department of Bacterial Genetics, Miecznikowa 1, 02-096 Warsaw, Poland. Phone: 48 22 5541344. Fax: 48 22 5541404. E-mail: bartosik{at}biol.uw.edu.pl.

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Journal of Bacteriology, July 2003, p. 3753-3763, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3753-3763.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Bartosik, D., Putyrski, M., Dziewit, L., Malewska, E., Szymanik, M., Jagiello, E., Lukasik, J., Baj, J. (2008). Transposable Modules Generated by a Single Copy of Insertion Sequence ISPme1 and Their Influence on Structure and Evolution of Natural Plasmids of Paracoccus methylutens DM12. J. Bacteriol. 190: 3306-3313 [Abstract] [Full Text]  
• Mikosa, M., Sochacka-Pietal, M., Baj, J., Bartosik, D. (2006). Identification of a transposable genomic island of Paracoccus pantotrophus DSM 11072 by its transposition to a novel entrapment vector pMMB2.. Microbiology 152: 1063-1073 [Abstract] [Full Text]  
• Bartosik, D., Szymanik, M., Baj, J. (2003). Identification and Distribution of Insertion Sequences of Paracoccus solventivorans. Appl. Environ. Microbiol. 69: 7002-7008 [Abstract] [Full Text]