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Journal of Bacteriology, August 2003, p. 4620-4625, Vol. 185, No. 15
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.15.4620-4625.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Biochemical Function of msl5 (pks8 plus pks17) in Mycobacterium tuberculosis H37Rv: Biosynthesis of Monomethyl Branched Unsaturated Fatty Acids

Vinod S. Dubey,1 Tatiana D. Sirakova,1 Michael H. Cynamon,2 and Pappachan E. Kolattukudy1*

Biomolecular Science Center and Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, Florida 32816,1 Department of Medicine, Veterans Affairs Medical Center, Syracuse, New York 132102

Received 20 March 2003/ Accepted 9 May 2003

We show that the disruption of one of the mycocerosic acid synthase (mas)-like genes, msl5 (pks8 plus pks17) in Mycobacterium tuberculosis H37Rv generates a mutant incapable of producing monomethyl branched unsaturated C16 to C20 fatty acids that are minor constituents of acyltrehaloses and sulfolipids. The msl5 mutation did not cause any significant change in the acyl lipid composition and also did not affect growth in culture, in mouse alveolar macrophage cell line MH-S, or in the murine lung.

* Corresponding author. Mailing address: Biomolecular Science Center, University of Central Florida, Bldg. 20, Rm. 136, Orlando, FL 32816. Phone: (407) 823-1206. Fax: (407) 823-0956. E-mail: pk{at}mail.ucf.edu.

Journal of Bacteriology, August 2003, p. 4620-4625, Vol. 185, No. 15
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.15.4620-4625.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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