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Journal of Bacteriology, August 2003, p. 4644-4647, Vol. 185, No. 15
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.15.4644-4647.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Transcriptional Expression of Escherichia coli Glutamate-Dependent Acid Resistance Genes gadA and gadBC in an hns rpoS Mutant{dagger}

Scott R. Waterman1* and P. L. C. Small2

Division of Human Immunology, Hanson Institute, Institute of Medical and Veterinary Science, Adelaide, South Australia, 5000, Australia,1 University of Tennessee—Knoxville, Knoxville, Tennessee 37996-08452

Received 10 February 2003/ Accepted 2 May 2003

Resistance to being killed by acidic environments with pH values lower than 3 is an important feature of both pathogenic and nonpathogenic Escherichia coli. The most potent E. coli acid resistance system utilizes two isoforms of glutamate decarboxylase encoded by gadA and gadB and a putative glutamate:{gamma}-aminobutyric acid antiporter encoded by gadC. The gad system is controlled by two repressors (H-NS and CRP), one activator (GadX), one repressor-activator (GadW), and two sigma factors ({sigma}S and {sigma}70). In contrast to results of previous reports, we demonstrate that gad transcription can be detected in an hns rpoS mutant strain of E. coli K-12, indicating that gad promoters can be initiated by {sigma}70 in the absence of H-NS.

* Corresponding author. Mailing address: Division of Human Immunology, Hanson Institute, IMVS, Adelaide, SA 5000, Australia. Phone: 61-8-8222-3297. Fax: 61-8-8232-4092. E-mail: scott.waterman{at}imvs.sa.gov.au.

{dagger} Dedicated to the loving memory of Geoff Banks.

Journal of Bacteriology, August 2003, p. 4644-4647, Vol. 185, No. 15
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.15.4644-4647.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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