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Journal of Bacteriology, August 2003, p. 4948-4955, Vol. 185, No. 16
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.16.4948-4955.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mapping the MinE Site Involved in Interaction with the MinD Division Site Selection Protein of Escherichia coli

Lu-Yan Ma,1 Glenn King,1,2 and Lawrence Rothfield1*

Departments of Microbiology,1 Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 060322

Received 31 January 2003/ Accepted 2 May 2003

Interactions between the MinD and MinE proteins are required for proper placement of the Escherichia coli division septum. The site within MinE that is required for interaction with MinD was mapped by studying the effects of site-directed minE mutations on MinD-MinE interactions in yeast two-hybrid and three-hybrid experiments. This confirmed that the MinE N-terminal domain is responsible for the interaction of MinE with MinD. Mutations that interfered with the interaction defined an extended surface on one face of the {alpha}-helical region of the MinE N-terminal domain, consistent with the idea that the MinE-MinD interaction involves formation of a coiled-coil structure by interaction with a complementary helical surface within MinD.

* Corresponding author. Mailing address: Department of Microbiology, University of Connecticut Health Center, Farmington, CT 06032. Phone: (860) 679-3581. Fax: (860) 679-1239. E-mail: lroth{at}neuron.uchc.edu.

Journal of Bacteriology, August 2003, p. 4948-4955, Vol. 185, No. 16
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.16.4948-4955.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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