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Journal of Bacteriology, August 2003, p. 5003-5006, Vol. 185, No. 16
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.16.5003-5006.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mutational Analysis of Cell Wall Biosynthesis in Mycobacterium avium

Jean-Pierre Laurent, Kirsten Hauge, Kellie Burnside, and Gerard Cangelosi*

Seattle Biomedical Research Institute, Seattle, Washington 98109

Received 22 April 2003/ Accepted 12 May 2003

The cell wall of the environmental pathogen Mycobacterium avium is important to its virulence and intrinsic antimicrobial resistance. To identify genes involved in cell wall biosynthesis, "transposome" insertion libraries were screened for mutants with altered colony morphology on medium containing the lipoprotein stain Congo red. Nineteen such mutants were isolated and mapped, including 10 with insertions in a functional island of cell wall biosynthetic genes that spans approximately 40 kb of the M. avium genome.

* Corresponding author. Mailing address: Seattle Biomedical Research Institute, 4 Nickerson St., Seattle, WA 98109. Phone: (206) 284-8846, ext. 313. Fax: (206) 284-0313. E-mail: gcang{at}sbri.org.

Journal of Bacteriology, August 2003, p. 5003-5006, Vol. 185, No. 16
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.16.5003-5006.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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