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Journal of Bacteriology, September 2003, p. 5295-5300, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5295-5300.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Purification and Characterization of the Flagellar Basal Body of Rhodobacter sphaeroides

K. Kobayashi,1,{dagger} T. Saitoh,1 D. S. H. Shah,2,{ddagger} K. Ohnishi,3 I. G. Goodfellow,2,§ R. E. Sockett,2 and S.-I. Aizawa1*

Department of Biosciences, Teikyo University, 1-1 Toyosatodai, Utsunomiya 320-8551,1 Institute of Genetics, Kochi University, 200 Monobe, Nangoku 783-8502, Japan,3 Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, United Kingdom2

Received 2 December 2002/ Accepted 12 June 2003

Flagellar hook-basal body (HBB) complexes were purified from Rhodobacter sphaeroides. The HBB was more acid labile but more heat stable than that of Salmonella species, and protein identification revealed that HBB components were expressed only from one of the two sets of flagellar gene clusters on the R. sphaeroides genome, under the heterotrophic growth conditions tested here.

* Corresponding author. Present address: CREST "Soft Nano-Machine Project," c/o Kochi Sangyo, 1064-18 Takahori, Hirata, Takanezawa, Shioya-gun, Tochigi 329-1206, Japan. Phone and fax: 81-28-676-8510. E-mail: aizawa{at}softnano.org.

{dagger} Present address: Department of Anatomy and Neuroscience, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan.

{ddagger} Present address: Orla Protein Technologies Ltd., Newcastle-upon-Tyne NE1 7RU, United Kingdom.

§ Present address: School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, United Kingdom.

Journal of Bacteriology, September 2003, p. 5295-5300, Vol. 185, No. 17
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.17.5295-5300.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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