This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rios-Velazquez, C.
Right arrow Articles by Donohue, T. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rios-Velazquez, C.
Right arrow Articles by Donohue, T. J.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2003, p. 422-431, Vol. 185, No. 2
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.2.422-431.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Features of Rhodobacter sphaeroides CcmFH

Carlos Rios-Velazquez, Ryan Coller, and Timothy J. Donohue*

Department of Bacteriology, University of Wisconsin—Madison, Madison, Wisconsin 53706

Received 30 July 2002/ Accepted 15 October 2002

In this study, the in vivo function and properties of two cytochrome c maturation proteins, CcmF and CcmH from Rhodobacter sphaeroides, were analyzed. Strains lacking CcmH or both CcmF and CcmH are unable to grow under anaerobic conditions where c-type cytochromes are required, demonstrating their critical role in the assembly of these electron carriers. Consistent with this observation, strains lacking both CcmF and CcmH are deficient in c-type cytochromes when assayed under permissive growth conditions. In contrast, under permissive growth conditions, strains lacking only CcmH contain several soluble and membrane-bound c-type cytochromes, albeit at reduced levels, suggesting that this bacterium has a CcmH-independent route for their maturation. In addition, the function of CcmH that is needed to support anaerobic growth can be replaced by adding cysteine or cystine to growth media. The ability of exogenous thiol compounds to replace CcmH provides the first physiological evidence for a role of this protein in thiol chemistry during c-type cytochrome maturation. The properties of R. sphaeroides cells containing translational fusions between CcmF and CcmH and either Escherichia coli alkaline phosphatase or ß-galactosidase suggest that they are each integral cytoplasmic membrane proteins with their presumed catalytic domains facing the periplasm. Analysis of CcmH shows that it is synthesized as a higher-molecular-weight precursor protein with an N-terminal signal sequence.

* Corresponding author. Mailing address: University of Wisconsin—Madison, Department of Bacteriology, 312 E.B. Fred Hall, 1550 Linden Dr., Madison, WI 53706. Phone: (608) 262-4663. Fax: (608) 262-9865. E-mail: tdonohue{at}bact.wisc.edu.

Journal of Bacteriology, January 2003, p. 422-431, Vol. 185, No. 2
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.2.422-431.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kranz, R. G., Richard-Fogal, C., Taylor, J.-S., Frawley, E. R. (2009). Cytochrome c Biogenesis: Mechanisms for Covalent Modifications and Trafficking of Heme and for Heme-Iron Redox Control. Microbiol. Mol. Biol. Rev. 73: 510-528 [Abstract] [Full Text]  
  • Rayapuram, N., Hagenmuller, J., Grienenberger, J. M., Bonnard, G., Giege, P. (2008). The Three Mitochondrial Encoded CcmF Proteins Form a Complex That Interacts with CCMH and c-Type Apocytochromes in Arabidopsis. J. Biol. Chem. 283: 25200-25208 [Abstract] [Full Text]  
  • Di Matteo, A., Gianni, S., Schinina, M. E., Giorgi, A., Altieri, F., Calosci, N., Brunori, M., Travaglini-Allocatelli, C. (2007). A Strategic Protein in Cytochrome c Maturation: THREE-DIMENSIONAL STRUCTURE OF CcmH AND BINDING TO APOCYTOCHROME c. J. Biol. Chem. 282: 27012-27019 [Abstract] [Full Text]  
  • Meyer, E. H., Giege, P., Gelhaye, E., Rayapuram, N., Ahuja, U., Thony-Meyer, L., Grienenberger, J.-M., Bonnard, G. (2005). AtCCMH, an essential component of the c-type cytochrome maturation pathway in Arabidopsis mitochondria, interacts with apocytochrome c. Proc. Natl. Acad. Sci. USA 102: 16113-16118 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»