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Journal of Bacteriology, January 2003, p. 669-673, Vol. 185, No. 2
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.2.669-673.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Novel Methylotrophy Genes of Methylobacterium extorquens AM1 Identified by using Transposon Mutagenesis Including a Putative Dihydromethanopterin Reductase

Christopher J. Marx,1 Brooke N. O'Brien,1 Jennifer Breezee,1 and Mary E. Lidstrom1,2*

Departments of Microbiology,1 Chemical Engineering, University of Washington, Seattle, Washington 98195-17502

Received 26 August 2002/ Accepted 16 October 2002

Ten novel methylotrophy genes of the facultative methylotroph Methylobacterium extorquens AM1 were identified from a transposon mutagenesis screen. One of these genes encodes a product having identity with dihydrofolate reductase (DHFR). This mutant has a C1-defective and methanol-sensitive phenotype that has previously only been observed for strains defective in tetrahydromethanopterin (H4MPT)-dependent formaldehyde oxidation. These results suggest that this gene, dmrA, may encode dihydromethanopterin reductase, an activity analogous to that of DHFR that is required for the final step of H4MPT biosynthesis.

* Corresponding author. Mailing address: Department of Chemical Engineering, University of Washington, Box 351750, Seattle, WA 98195-1750. Phone: (206) 616-5282. Fax: (206) 616-5721. E-mail: lidstrom{at}u.washington.edu.

Journal of Bacteriology, January 2003, p. 669-673, Vol. 185, No. 2
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.2.669-673.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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