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Journal of Bacteriology, October 2003, p. 6195-6198, Vol. 185, No. 20
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.20.6195-6198.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mutations Affecting DNA-Binding Activity of the MexR Repressor of mexR-mexA-mexB-oprM Operon Expression

Kohjiro Saito, Hiroyuki Akama, Eisaku Yoshihara, and Taiji Nakae*

Department of Molecular Life Science, Tokai University School of Medicine, Isehara 259-1193, Japan

Received 13 June 2003/ Accepted 29 July 2003

We have isolated 25 MexR mutants that retained their dimerizing ability but were unable to bind mexOP DNA. Surprisingly, 20 mutations were located in the hydrophobic core region at {alpha}4, W1, {alpha}2, {alpha}3, and ß2, and only 3 were in positively charged residues. These results verified that DNA binding is mediated by distinct regions of MexR and showed the importance of the hydrophobic core region of the DNA-binding domain.

* Corresponding author. Mailing address: Department of Molecular Life Science, Tokai University School of Medicine, Isehara 259-1193, Japan. Phone: 81-463-93-5436. Fax. 81-463-93-5437. E-mail: nakae{at}is.icc.u-tokai.ac.jp.

Journal of Bacteriology, October 2003, p. 6195-6198, Vol. 185, No. 20
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.20.6195-6198.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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