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Journal of Bacteriology, November 2003, p. 6678-6685, Vol. 185, No. 22
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.22.6678-6685.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Product of a Developmental Gene, crgA, That Coordinates Reproductive Growth in Streptomyces Belongs to a Novel Family of Small Actinomycete-Specific Proteins

Ricardo Del Sol, Andrew Pitman,{dagger} Paul Herron, and Paul Dyson*

Molecular Biology Research Group, School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, United Kingdom

Received 22 May 2003/ Accepted 26 August 2003

On solid media, the reproductive growth of Streptomyces involves antibiotic biosynthesis coincident with the erection of filamentous aerial hyphae. Following cessation of growth of an aerial hypha, multiple septation occurs at the tip to form a chain of unigenomic spores. A gene, crgA, that coordinates several aspects of this reproductive growth is described. The gene product is representative of a well-conserved family of small actinomycete proteins with two C-terminal hydrophobic-potential membrane-spanning segments. In Streptomyces avermitilis, crgA is required for sporulation, and inactivation of the gene abolished most sporulation septation in aerial hyphae. Disruption of the orthologous gene in Streptomyces coelicolor indicates that whereas CrgA is not essential for sporulation in this species, during growth on glucose-containing media, it influences the timing of the onset of reproductive growth, with precocious erection of aerial hyphae and antibiotic production by the mutant. Moreover, CrgA subsequently acts to inhibit sporulation septation prior to growth arrest of aerial hyphae. Overexpression of CrgA in S. coelicolor, uncoupling any nutritional and growth phase-dependent regulation, results in growth of nonseptated aerial hyphae on all media tested, consistent with a role for the protein in inhibiting sporulation septation.

* Corresponding author. Mailing address: Molecular Biology Research Group, School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, United Kingdom. Phone: (44) 1792 295667. Fax: (44) 1792 295447. E-mail: p.j.dyson{at}swansea.ac.uk.

{dagger} Present address: Department of Biological Science, University of the West of England, Frenchay Campus, Coldharbour Ln., Bristol BS16 1QY, United Kingdom.

Journal of Bacteriology, November 2003, p. 6678-6685, Vol. 185, No. 22
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.22.6678-6685.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mistry, B. V., Del Sol, R., Wright, C., Findlay, K., Dyson, P. (2008). FtsW Is a Dispensable Cell Division Protein Required for Z-Ring Stabilization during Sporulation Septation in Streptomyces coelicolor. J. Bacteriol. 190: 5555-5566 [Abstract] [Full Text]  
  • Jones, G., Dyson, P. (2006). Evolution of Transmembrane Protein Kinases Implicated in Coordinating Remodeling of Gram-Positive Peptidoglycan: Inside versus Outside. J. Bacteriol. 188: 7470-7476 [Abstract] [Full Text]  
  • Del Sol, R., Mullins, J. G. L., Grantcharova, N., Flardh, K., Dyson, P. (2006). Influence of CrgA on Assembly of the Cell Division Protein FtsZ during Development of Streptomyces coelicolor. J. Bacteriol. 188: 1540-1550 [Abstract] [Full Text]  
  • Grantcharova, N., Lustig, U., Flardh, K. (2005). Dynamics of FtsZ Assembly during Sporulation in Streptomyces coelicolor A3(2). J. Bacteriol. 187: 3227-3237 [Abstract] [Full Text]  


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