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Journal of Bacteriology, November 2003, p. 6719-6722, Vol. 185, No. 22
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.22.6719-6722.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Translocon Pulling of Nascent SecM Controls the Duration of Its Translational Pause and Secretion-Responsive secA Regulation

Martha E. Butkus, Lucia B. Prundeanu, and Donald B. Oliver*

Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, Connecticut 06459

Received 28 May 2003/ Accepted 27 August 2003

SecA is an ATPase and motor protein that drives protein translocation across the bacterial plasma membrane. In Escherichia coli SecA levels are regulated by the secretion needs of the cell utilizing secM, which encodes a secreted protein. Previous studies demonstrated that this regulation requires a translational pause within secM, whose duration regulates the accessibility of the secA Shine-Dalgarno sequence on secM secA mRNA. Here we provide evidence that translocon pulling of nascent SecM is what regulates the duration of the secM translational pause, and thus secA expression levels, thereby providing direct support for this model.

* Corresponding author. Mailing address: Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06459. Phone: (860) 685-3556. Fax: (860) 685-2141. E-mail: doliver{at}wesleyan.edu.

Journal of Bacteriology, November 2003, p. 6719-6722, Vol. 185, No. 22
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.22.6719-6722.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Garza-Sanchez, F., Janssen, B. D., Hayes, C. S. (2006). Prolyl-tRNAPro in the A-site of SecM-arrested Ribosomes Inhibits the Recruitment of Transfer-messenger RNA. J. Biol. Chem. 281: 34258-34268 [Abstract] [Full Text]  
  • Nakatogawa, H., Murakami, A., Mori, H., Ito, K. (2005). SecM facilitates translocase function of SecA by localizing its biosynthesis. Genes Dev. 19: 436-444 [Abstract] [Full Text]  
  • Collier, J., Bohn, C., Bouloc, P. (2004). SsrA Tagging of Escherichia coli SecM at Its Translation Arrest Sequence. J. Biol. Chem. 279: 54193-54201 [Abstract] [Full Text]  
  • Murakami, A., Nakatogawa, H., Ito, K. (2004). Translation arrest of SecM is essential for the basal and regulated expression of SecA. Proc. Natl. Acad. Sci. USA 101: 12330-12335 [Abstract] [Full Text]  


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