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Journal of Bacteriology, December 2003, p. 7001-7007, Vol. 185, No. 23
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.23.7001-7007.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Quorum Sensing Negative Regulators EsaR and ExpREcc, Homologues within the LuxR Family, Retain the Ability To Function as Activators of Transcription

Susanne B. von Bodman,1 Jessica K. Ball,2 Marie A. Faini,2 Carmen M. Herrera,1 Timothy D. Minogue,1 Mark L. Urbanowski,3 and Ann M. Stevens2*

Department of Plant Science, University of Connecticut, Storrs, Connecticut 06269,1 Department of Biology, Virginia Tech, Blacksburg, Virginia 24061,2 Department of Microbiology, University of Iowa, Iowa City, Iowa 522423

Received 1 July 2003/ Accepted 10 September 2003

Most LuxR homologues function as activators of transcription during the process of quorum sensing, but a few, including EsaR and ExpREcc, negatively impact gene expression. The LuxR-activated luxI promoter and LuxR binding site, the lux box, were used in artificial contexts to assess the potential for transcriptional activation and DNA binding by EsaR and ExpREcc. Although the acyl-homoserine lactone responsiveness of both proteins is the opposite of that shown by most LuxR family members, EsaR and ExpREcc have preserved the ability to interact with RNA polymerase and activate transcription despite their low affinity for the lux box DNA.

* Corresponding author. Mailing address: 4036 Derring Hall, Department of Biology, Virginia Tech, Blacksburg, VA 24061. Phone: (540) 231-9378. Fax: (540) 231-9307. E-mail: ams{at}vt.edu.

Journal of Bacteriology, December 2003, p. 7001-7007, Vol. 185, No. 23
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.23.7001-7007.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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