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Journal of Bacteriology, December 2003, p. 7273-7278, Vol. 185, No. 24
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.24.7273-7278.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Streptomyces coelicolor Polynucleotide Phosphorylase Homologue, and Not the Putative Poly(A) Polymerase, Can Polyadenylate RNA

Björn Sohlberg,1 Jianqiang Huang,1 and Stanley N. Cohen1,2*

Department of Genetics,1 Department of Medicine, Stanford University School of Medicine, Stanford, California 94305-51202

Received 30 July 2003/ Accepted 17 September 2003

A protein containing a nucleotidyltransferase motif characteristic of poly(A) polymerases has been proposed to polyadenylate RNA in Streptomyces coelicolor (P. Bralley and G. H. Jones, Mol. Microbiol. 40:1155-1164, 2001). We show that this protein lacks poly(A) polymerase activity and is instead a tRNA nucleotidyltransferase that repairs CCA ends of tRNAs. In contrast, a Streptomyces coelicolor polynucleotide phosphorylase homologue that exhibits polyadenylation activity may account for the poly(A) tails found in this organism.

* Corresponding author. Mailing address: Department of Genetics, Rm. M-320, Stanford University School of Medicine, Stanford, CA 94305-5120. Phone: (650) 723-5315. Fax: (650) 725-1536. E-mail: sncohen{at}stanford.edu.

Journal of Bacteriology, December 2003, p. 7273-7278, Vol. 185, No. 24
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.24.7273-7278.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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