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Journal of Bacteriology, February 2003, p. 1027-1036, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.1027-1036.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Rhamnolipid Surfactant Production Affects Biofilm Architecture in Pseudomonas aeruginosa PAO1{dagger}

Mary E. Davey, Nicky C. Caiazza, and George A. O'Toole*

Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755

Received 10 June 2002/ Accepted 6 October 2002

In response to certain environmental signals, bacteria will differentiate from an independent free-living mode of growth and take up an interdependent surface-attached existence. These surface-attached microbial communities are known as biofilms. In flowing systems where nutrients are available, biofilms can develop into elaborate three-dimensional structures. The development of biofilm architecture, particularly the spatial arrangement of colonies within the matrix and the open areas surrounding the colonies, is thought to be fundamental to the function of these complex communities. Here we report a new role for rhamnolipid surfactants produced by the opportunistic pathogen Pseudomonas aeruginosa in the maintenance of biofilm architecture. Biofilms produced by mutants deficient in rhamnolipid synthesis do not maintain the noncolonized channels surrounding macrocolonies. We provide evidence that surfactants may be able to maintain open channels by affecting cell-cell interactions and the attachment of bacterial cells to surfaces. The induced synthesis of rhamnolipids during the later stages of biofilm development (when cell density is high) implies an active mechanism whereby the bacteria exploit intercellular interaction and communication to actively maintain these channels. We propose that the maintenance of biofilm architecture represents a previously unrecognized step in the development of these microbial communities.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Dartmouth Medical School, Rm. 202, Vail Building, North College St., Hanover, NH 03755. Phone: (603) 650-1248. Fax: (603) 650-1318. E-mail: georgeo{at}dartmouth.edu.

{dagger} For a commentary on this article, see page 699 in this issue.

Journal of Bacteriology, February 2003, p. 1027-1036, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.1027-1036.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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