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Journal of Bacteriology, February 2003, p. 1120-1124, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.1120-1124.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Chromosomal Arm Replacement in Streptomyces griseus

Tetsuya Uchida, Mariko Miyawaki, and Haruyasu Kinashi*

Department of Molecular Biotechnology, Hiroshima University, Higashi-Hiroshima 739-8530, Japan

Received 7 August 2002/ Accepted 5 November 2002

UV irradiation of Streptomyces griseus 2247 yielded a new chromosomal deletion mutant, MM9. Restriction and sequencing analysis revealed that homologous recombination between two similar lipoprotein-like open reading frames, which are located 450 and 250 kb from the left and right ends, respectively, caused chromosomal arm replacement. As a result, new 450-kb terminal inverted repeats (TIRs) were formed in place of the original 24-kb TIRs. Frequent homologous recombinations in Streptomyces strains suggest that telomere deletions can usually be repaired by recombinational DNA repair functioning between the intact and deleted TIR sequences on the same chromosome.

* Corresponding author. Mailing address: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan. Phone: 81-824-24-7869. Fax: 81-824-24-7869. E-mail: kinashi{at}hiroshima-u.ac.jp.

Journal of Bacteriology, February 2003, p. 1120-1124, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.1120-1124.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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