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Journal of Bacteriology, February 2003, p. 948-956, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.948-956.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A SecE Mutation That Modulates SecY-SecE Translocase Assembly, Identified as a Specific Suppressor of SecY Defects

Hiroyuki Mori, Yoshinori Akiyama, and Koreaki Ito*

Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan

Received 30 September 2002/ Accepted 4 November 2002

The SecY39(Cs) (cold-sensitive) alteration of Arg357 results in a defect of translocation initiation. As a means to dissect the Sec translocation machinery, we isolated mutations that act as suppressors of the secY39 defect. A specific secE mutation, designated secE105, was thus isolated. This mutation proved to be identical with the prlG2 mutation and to suppress a number of cold-sensitive secY mutations. However, other prlG mutations did not effectively suppress the secY defects. Evidence indicates that the Ser105-to-Pro alteration in the C-terminal transmembrane segment of SecE weakens SecY-SecE association. In vitro analyses showed that the SecE(S105P) alteration preferentially stimulates the initial phase of translocation. It is suggested that the S105P alteration affects the SecYEG channel such that it is more prone to open and to accept the translocation initiation domain of a preprotein molecule.

* Corresponding author. Mailing address: Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan. Phone: 81-75-751-4015. Fax: 81-75-771-5699. E-mail: kito{at}virus.kyoto-u.ac.jp.

Journal of Bacteriology, February 2003, p. 948-956, Vol. 185, No. 3
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.3.948-956.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mori, H., Ito, K. (2006). Different modes of SecY-SecA interactions revealed by site-directed in vivo photo-cross-linking. Proc. Natl. Acad. Sci. USA 103: 16159-16164 [Abstract] [Full Text]  
  • Mori, H., Shimokawa, N., Satoh, Y., Ito, K. (2004). Mutational Analysis of Transmembrane Regions 3 and 4 of SecY, a Central Component of Protein Translocase. J. Bacteriol. 186: 3960-3969 [Abstract] [Full Text]  


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