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Journal of Bacteriology, February 2003, p. 1459-1461, Vol. 185, No. 4
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.4.1459-1461.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

2-Aminopurine Allows Interspecies Recombination by a Reversible Inactivation of the Escherichia coli Mismatch Repair System

Ivan Matic,1* Ana Babic,1,2 and Miroslav Radman1

INSERM U571,1 MIXIS France SA, Faculté de Médecine, Necker-Enfants Malades, Université René Descartes, 75730 Paris Cedex 15, France2

Received 3 September 2002/ Accepted 22 November 2002

2-Aminopurine treatment of Escherichia coli induces a reversible phenotype of DNA mismatch repair deficiency. This transient phenotype results in a 300-fold increase in the frequency of interspecies conjugational recombination with a Salmonella enterica serovar Typhimurium Hfr donor. This method can be used for the generation of biodiversity by allowing recombination between diverged genes and genomes.

* Corresponding author. Mailing address: INSERM U571, Faculté de Médecine Necker-Enfants Malades, Université Paris V, 156 rue Vaugirard, 75730 Paris Cedex 15, France. Phone: (33) 1 40 61 53 25. Fax: (33) 1 40 61 53 22. E-mail: matic{at}necker.fr.

Journal of Bacteriology, February 2003, p. 1459-1461, Vol. 185, No. 4
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.4.1459-1461.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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  • Velappan, N., Sblattero, D., Chasteen, L., Pavlik, P., Bradbury, A. R.M. (2007). Plasmid incompatibility: more compatible than previously thought?. Protein Eng Des Sel 20: 309-313 [Abstract] [Full Text]  
  • Costantino, N., Court, D. L. (2003). Enhanced levels of {lambda} Red-mediated recombinants in mismatch repair mutants. Proc. Natl. Acad. Sci. USA 100: 15748-15753 [Abstract] [Full Text]  


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