Journal of Bacteriology, March 2003, p. 1749-1756, Vol. 185, No. 5
0021-9193/03/$08.00+0 DOI: 10.1128/JB.185.5.1749-1756.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Microbiological Resources and Technology Laboratories,1 Health and Bioscience Laboratories, Meiji Seika Kaisha, Ltd., Saitama 350-0289, Japan2
Received 23 September 2002/ Accepted 25 November 2002
Three endoglucanase genes, designated the rce1, rce2, and rce3 genes, were isolated from Rhizopus oryzae as the first cellulase genes from the subdivision Zygomycota. All the amino acid sequences deduced from the rce1, rce2, and rce3 genes consisted of three distinct domains: cellulose binding domains, linker domains, and catalytic domains belonging to glycosyl hydrolase family 45. The rce3 gene had two tandem repeated sequences of cellulose binding domains, while rce1 and rce2 had only one. rce1, rce2, and rce3 had various lengths of linker sequences.
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