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Journal of Bacteriology, March 2003, p. 2017-2021, Vol. 185, No. 6
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.6.2017-2021.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Definition of the Escherichia coli MC4100 Genome by Use of a DNA Array

Joseph E. Peters,{dagger} Timothy E. Thate, and Nancy L. Craig*

Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland

Received 5 July 2002/ Accepted 10 December 2002

We have used an Escherichia coli K-12 whole-genome array based on the DNA sequence of strain MG1655 as a tool to identify deletions in another E. coli K-12 strain, MC4100, by probing the array with labeled chromosomal DNA. Despite the continued widespread use of MC4100 as an experimental system, the specific genetic relationship of this strain to the sequenced K-12 derivative MG1655 has not been resolved. MC4100 was found to contain four deletions, ranging from 1 to 97 kb in size. The exact nature of three of the deletions was previously unresolved, and the fourth deletion was altogether unknown.

* Corresponding author. Mailing address: Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 North Wolfe St., Baltimore, MD 21205. Phone: (410) 955-3933. Fax: (410) 955-0831. E-mail: ncraig{at}jhmi.edu.

{dagger} Present address: Department of Microbiology, Cornell University, Ithaca, New York 14853.

Journal of Bacteriology, March 2003, p. 2017-2021, Vol. 185, No. 6
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.6.2017-2021.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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