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Journal of Bacteriology, March 2003, p. 2042-2045, Vol. 185, No. 6
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.6.2042-2045.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Effects of ISSo2 Insertions in Structural and Regulatory Genes of the Trimethylamine Oxide Reductase of Shewanella oneidensis

Christophe Bordi, Chantal Iobbi-Nivol, Vincent Méjean,* and Jean-Claude Patte

Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, 13402 Marseille Cedex 20, France

Received 11 November 2002/ Accepted 26 December 2002

We have isolated three Shewanella oneidensis mutants specifically impaired in trimethylamine oxide (TMAO) respiration. The mutations arose from insertions of an ISSo2 element into torA, torR, and torS, encoding, respectively, the TMAO reductase TorA, the response regulator TorR, and the sensor TorS. Although TorA is not the sole enzyme reducing TMAO in S. oneidensis, growth analysis showed that it is the main respiratory TMAO reductase. Use of a plasmid-borne torE'-lacZ fusion confirmed that the TorS-TorR phosphorelay mediates TMAO induction of the torECAD operon.

* Corresponding author. Mailing address: Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, 31, chemin Joseph Aiguier, 13402 Marseille Cedex 20, France. Phone: (33) 4 91 16 40 32. Fax: (33) 4 91 71 89 14. E-mail: mejean{at}ibsm.cnrs-mrs.fr.

Journal of Bacteriology, March 2003, p. 2042-2045, Vol. 185, No. 6
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.6.2042-2045.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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