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Journal of Bacteriology, April 2003, p. 2236-2242, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2236-2242.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Differential Expression of the Escherichia coli Autoaggregation Factor Antigen 43

Mark A. Schembri, Louise Hjerrild, Morten Gjermansen, and Per Klemm*

Microbial Adhesion Group, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark

Received 2 October 2002/ Accepted 26 December 2002

Antigen 43 (Ag43) is a self-recognizing surface adhesin found in most Escherichia coli strains. Due to its excellent cell-to-cell aggregation characteristics, Ag43 expression confers clumping and fluffing of cells and promotes biofilm formation. Ag43 expression is repressed by the cellular redox sensor OxyR. Here we used mutant versions of OxyR that are locked in either the reduced or the oxidized form as well as the addition of a simple redox-changing chemical to show that the redox state of OxyR influences Ag43 expression. Furthermore, the redox state of OxyR influences the biofilm-forming potential of E. coli. Finally, we demonstrated that Ag43-mediated cell aggregation confers significant protection against hydrogen peroxide killing.

* Corresponding author. Mailing address: Section of Molecular Microbiology, BioCentrum-DTU, Bldg. 301, Technical University of Denmark, DK-2800 Lyngby, Denmark. Phone: 45 45 25 25 06. Fax: 45 45 93 28 09. E-mail: per.klemm{at}biocentrum.dtu.dk.

Journal of Bacteriology, April 2003, p. 2236-2242, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2236-2242.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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