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Journal of Bacteriology, April 2003, p. 2387-2391, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2387-2391.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Analysis of the Ferric Citrate Transport Gene Promoter of Escherichia coli

Sabine Enz, Susanne Mahren, Claudia Menzel, and Volkmar Braun*

Mikrobiologie/Membranphysiologie, Universität Tübingen; D-72076 Tübingen, Germany

Received 6 December 2002/ Accepted 2 January 2003

FecI, an extracytoplasmic-function {sigma} factor, is required for initiation of transcription of the ferric citrate transport genes. A mutational analysis of the fecA promoter revealed that the nonconserved -10 region and a downstream regulatory element are important for fecA promoter activity. However, nucleotide substitutions in the well-conserved -35 region also have an effect on the fecA promoter activity. Titration of FecI suggests that the FecI-RNA polymerase holoenzyme does not bind strongly to the downstream regulatory element, which is therefore probably involved in a subsequent step of transcription initiation.

* Corresponding author. Mailing address: Mikrobiologie/Membranphysiologie, Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany. Phone: 49 7071 2972096. Fax: 49 7071 295843. E-mail: volkmar.braun{at}mikrobio.uni-tuebingen.de.

Journal of Bacteriology, April 2003, p. 2387-2391, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2387-2391.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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