This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Han, S. O.
Right arrow Articles by Doi, R. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Han, S. O.
Right arrow Articles by Doi, R. H.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2003, p. 2520-2527, Vol. 185, No. 8
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.8.2520-2527.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Transcription of Clostridium cellulovorans Cellulosomal Cellulase and Hemicellulase Genes

Sung Ok Han,1 Hideaki Yukawa,2 Masayuki Inui,2 and Roy H. Doi1*

Section of Molecular and Cellular Biology, University of California, Davis, California 95616,1 Molecular Microbiology and Genetics Lab, RITE Institute, Kyoto 619-0292, Japan2

Received 10 January 2003/ Accepted 3 February 2003

Transcription of the cellulosomal cellulase/hemicellulase genes of Clostridium cellulovorans has been investigated by Northern blot, reverse transcriptase PCR (RT-PCR), primer extension, and S1 nuclease analysis. Northern hybridizations revealed that the cellulosomal cbpA gene cluster is transcribed as polycistronic mRNAs of 8 and 12 kb. The 8-kb mRNA coded for cbpA and exgS, and the 12-kb mRNA coded for cbpA, exgS, engH, and engK. The sizes of the mRNAs were about 3 kb for engE, 1.8 kb for manA, 2.7 kb for xynA, and 4 kb for pelA, indicating monocistronic transcription of these genes. Primer extension and S1 nuclease analysis of C. cellulovorans RNA showed that the transcriptional start sites of cbpA, engE, manA, and hbpA were located 233, 97, 64, and 61 bp upstream from the first nucleotide of each of the respective translation initiation codons. Alignment of the cbpA, engE, manA, and hbpA promoter regions provided evidence for highly conserved sequences that exhibited strong similarity to the {sigma}A consensus promoter sequences of gram-positive bacteria.

* Corresponding author. Mailing address: Section of Molecular and Cellular Biology, University of California, Davis, CA 95616. Phone: (530) 752-3191. Fax: (530) 752-3085. E-mail: rhdoi{at}ucdavis.edu.

Journal of Bacteriology, April 2003, p. 2520-2527, Vol. 185, No. 8
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.8.2520-2527.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Maamar, H., Abdou, L., Boileau, C., Valette, O., Tardif, C. (2006). Transcriptional Analysis of the cip-cel Gene Cluster from Clostridium cellulolyticum.. J. Bacteriol. 188: 2614-2624 [Abstract] [Full Text]  
  • Han, S. O., Yukawa, H., Inui, M., Doi, R. H. (2005). Molecular Cloning and Transcriptional and Expression Analysis of engO, Encoding a New Noncellulosomal Family 9 Enzyme, from Clostridium cellulovorans. J. Bacteriol. 187: 4884-4889 [Abstract] [Full Text]  
  • Han, S. O., Yukawa, H., Inui, M., Doi, R. H. (2005). Effect of carbon source on the cellulosomal subpopulations of Clostridium cellulovorans. Microbiology 151: 1491-1497 [Abstract] [Full Text]  
  • Demain, A. L., Newcomb, M., Wu, J. H. D. (2005). Cellulase, Clostridia, and Ethanol. Microbiol. Mol. Biol. Rev. 69: 124-154 [Abstract] [Full Text]  
  • Zhang, Y.-H. P., Lynd, L. R. (2005). Regulation of Cellulase Synthesis in Batch and Continuous Cultures of Clostridium thermocellum. J. Bacteriol. 187: 99-106 [Abstract] [Full Text]  
  • Han, S. O., Yukawa, H., Inui, M., Doi, R. H. (2004). Isolation and Expression of the xynB Gene and Its Product, XynB, a Consistent Component of the Clostridium cellulovorans Cellulosome. J. Bacteriol. 186: 8347-8355 [Abstract] [Full Text]  
  • Han, S. O., Cho, H.-Y., Yukawa, H., Inui, M., Doi, R. H. (2004). Regulation of Expression of Cellulosomes and Noncellulosomal (Hemi)Cellulolytic Enzymes in Clostridium cellulovorans during Growth on Different Carbon Sources. J. Bacteriol. 186: 4218-4227 [Abstract] [Full Text]  
  • Doi, R. H., Kosugi, A., Murashima, K., Tamaru, Y., Han, S. O. (2003). Cellulosomes from Mesophilic Bacteria. J. Bacteriol. 185: 5907-5914 [Full Text]  
  • Han, S. O., Yukawa, H., Inui, M., Doi, R. H. (2003). Regulation of Expression of Cellulosomal Cellulase and Hemicellulase Genes in Clostridium cellulovorans. J. Bacteriol. 185: 6067-6075 [Abstract] [Full Text]  
  • Dror, T. W., Rolider, A., Bayer, E. A., Lamed, R., Shoham, Y. (2003). Regulation of Expression of Scaffoldin-Related Genes in Clostridium thermocellum. J. Bacteriol. 185: 5109-5116 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»