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Journal of Bacteriology, January 2004, p. 240-243, Vol. 186, No. 1
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.1.240-243.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification of Residues of the Kid Toxin Involved in Autoregulation of the parD System

Marc Lemonnier, Sandra Santos-Sierra,{dagger} Consolación Pardo-Abarrio, and Ramón Díaz-Orejas*

Centro de Investigaciones Biológicas (CSIC), 28040 Madrid, Spain

Received 27 August 2003/ Accepted 30 September 2003

The toxin-antitoxin system parD (kis kid) of plasmid R1 is coregulated by the coordinated action of its two gene products. Here we describe the isolation and the in vivo characterization of three single-amino-acid changes in the Kid toxin, G4E, C74Y, and E91K, that affect the coregulatory activity but preserve the toxicity of the protein.

* Corresponding author. Mailing address: Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain. Phone: 3491-8373112. Fax: 3491-5630432. E-mail: ramondiaz{at}cib.csic.es.

{dagger} Present address: Institut für Biochemische Pharmakologie, Universität Innsbruck, A-6020 Innsbruck, Austria.

Journal of Bacteriology, January 2004, p. 240-243, Vol. 186, No. 1
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.1.240-243.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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