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Journal of Bacteriology, May 2004, p. 3015-3021, Vol. 186, No. 10
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.10.3015-3021.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

PhaQ, a New Class of Poly-ß-Hydroxybutyrate (PHB)-Responsive Repressor, Regulates phaQ and phaP (Phasin) Expression in Bacillus megaterium through Interaction with PHB

Tian-Ren Lee, Jer-Sheng Lin, Shih-Shin Wang, and Gwo-Chyuan Shaw*

Institute of Biochemistry, School of Life Science, National Yang-Ming University, Taipei, Taiwan, Republic of China

Received 31 October 2003/ Accepted 2 February 2004

Bacillus megaterium can produce poly-ß-hydroxybutyrate (PHB) as carbon and energy storage materials. We now report that the phaQ gene, which is located upstream of the phasin-encoding phaP gene, codes for a new class of transcriptional regulator that negatively controls expression of both phaQ and phaP. A PhaQ binding site that plays a role in this control has been identified by gel mobility shift assays and DNase I footprinting analysis. We have also provided evidence that PhaQ could sense the presence of PHB in vivo and that artificial PHB granules could inhibit the formation of PhaQ-DNA complex in vitro by binding to PhaQ directly. These suggest that PhaQ is a PHB-responsive repressor.

* Corresponding author. Mailing address: Institute of Biochemistry, School of Life Science, National Yang-Ming University, Taipei 112, Taiwan. Phone: 886-2-2826-7127. Fax: 886-2-2826-4843. E-mail: gcshaw{at}ym.edu.tw.

Journal of Bacteriology, May 2004, p. 3015-3021, Vol. 186, No. 10
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.10.3015-3021.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chen, H.-J., Pan, S.-C., Shaw, G.-C. (2009). Identification and Characterization of a Novel Intracellular Poly(3-Hydroxybutyrate) Depolymerase from Bacillus megaterium. Appl. Environ. Microbiol. 75: 5290-5299 [Abstract] [Full Text]  
  • Jendrossek, D. (2009). Polyhydroxyalkanoate Granules Are Complex Subcellular Organelles (Carbonosomes). J. Bacteriol. 191: 3195-3202 [Full Text]  
  • Tseng, C.-L., Chen, H.-J., Shaw, G.-C. (2006). Identification and Characterization of the Bacillus thuringiensis phaZ Gene, Encoding New Intracellular Poly-3-Hydroxybutyrate Depolymerase. J. Bacteriol. 188: 7592-7599 [Abstract] [Full Text]  


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