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Journal of Bacteriology, May 2004, p. 3270-3273, Vol. 186, No. 10
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.10.3270-3273.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of a Generalized Transducing Phage for Pseudomonas aeruginosa Strains PAO1 and PA14

Jonathan M. Budzik,1 William A. Rosche,2 Arne Rietsch,3 and George A. O'Toole4*

Dartmouth College and Department of Microbiology and Immunology,1 Dartmouth Medical School, Hanover, New Hampshire 03755,4 Faculty of Biological Science, The University of Tulsa, Tulsa, Oklahoma 74104,2 Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 021153

Received 29 October 2003/ Accepted 2 February 2004

A temperate, type IV pilus-dependent, double-stranded DNA bacteriophage named DMS3 was isolated from a clinical strain of Pseudomonas aeruginosa. A clear-plaque variant of this bacteriophage was isolated. DMS3 is capable of mediating generalized transduction within and between P. aeruginosa strains PA14 and PAO1, thus providing a useful tool for the genetic analysis of P. aeruginosa.

* Corresponding author. Mailing address: Dartmouth Medical School, Dept. of Microbiology & Immunology, Rm. 202 Vail Building, N. College St., Hanover, NH 03755. Phone: (603) 650-1248. Fax: (603) 650-1318. E-mail: georgeo{at}Dartmouth.edu.

Journal of Bacteriology, May 2004, p. 3270-3273, Vol. 186, No. 10
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.10.3270-3273.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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