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Journal of Bacteriology, June 2004, p. 3882-3888, Vol. 186, No. 12
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.12.3882-3888.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Control of Photosynthetic and High-Light-Responsive Genes by the Histidine Kinase DspA: Negative and Positive Regulation and Interactions between Signal Transduction Pathways

Hui-Yi Hsiao,1 Qingfang He,1* Lorraine G. van Waasbergen,2 and Arthur R. Grossman3

Department of Applied Science, University of Arkansas at Little Rock, Little Rock, Arkansas 72204,1 Department of Biology, University of Texas at Arlington, Arlington, Texas 76019,2 Department of Plant Biology, The Carnegie Institution of Washington, Stanford, California 943053

Received 16 July 2003/ Accepted 6 February 2004

We have deleted a gene for a sensor histidine kinase, dspA (or hik33), in the cyanobacterium Synechocystis sp. strain PCC6803. In low and moderate light, the mutant grew slowly under photoautotrophic conditions, with a doubling time of ~40 h, and had severely reduced photosynthetic oxygen evolution. When the mutant was maintained in low or moderate light in the presence of glucose, its growth rate was only somewhat lower than that of wild-type cells. However, the mutant was light sensitive and rapidly died in high light. Furthermore, levels of many transcripts encoding genes associated with photosynthesis were altered in the mutant relative to wild-type Synechocystis sp. strain PCC6803 both in low light and following exposure to high light. There was constitutive expression of several high-light-inducible genes, including hli, psbAIII, and gpx2; there was little increased accumulation of sodB mRNA in high light; and the cells failed to accumulate cpcBA and psaAB mRNAs in low light in the presence of glucose, although a normal decline in the levels of these mRNAs was observed during exposure to high light. These results suggest that DspA is involved in controlling sets of photosynthetic and high-light-responsive genes, either directly or indirectly. These and other results, some of which are presented in a companion paper (C.-J. Tu, J. Shrager, R. Burnap, B. L. Postier, and A. R. Grossman, J. Bacteriol. 186:3889-3902, 2004), suggest that DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions.

* Corresponding author. Mailing address: Department of Applied Science, University of Arkansas at Little Rock, Little Rock, AR 72204. Phone: (501) 569-8033. Fax: (501) 569-8020. E-mail: qfhe{at}ualr.edu.

Journal of Bacteriology, June 2004, p. 3882-3888, Vol. 186, No. 12
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.12.3882-3888.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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