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Journal of Bacteriology, June 2004, p. 3889-3902, Vol. 186, No. 12
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.12.3889-3902.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Consequences of a Deletion in dspA on Transcript Accumulation in Synechocystis sp. Strain PCC6803{dagger}

Chao-Jung Tu,1* Jeffrey Shrager,1 Robert L. Burnap,2 Bradley L. Postier,2 and Arthur R. Grossman1

Department of Plant Biology, The Carnegie Institution of Washington, Stanford, California 94305,1 Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma 740782

Received 23 July 2003/ Accepted 24 February 2004

A sensor histidine kinase of Synechococcus sp. strain PCC7942, designated nblS, was previously identified and shown to be critical for the acclimation of cells to high-light and nutrient limitation conditions and to influence the expression of a number of light-responsive genes. The nblS orthologue in Synechocystis sp. strain PCC6803 is designated dspA (also called hik33). We have generated a dspA null mutant and analyzed global gene expression in both the mutant and wild-type strains under high- and low-light conditions. The mutant is aberrant for the expression of many genes encoding proteins critical for photosynthesis, phosphate and carbon acquisition, and the amelioration of stress conditions. Furthermore, transcripts from a number of genes normally detected only during exposure of wild-type cells to high-light conditions become partially constitutive in the low-light-grown dspA mutant. Other genes for which transcripts decline upon exposure of wild-type cells to high light are already lower in the mutant during growth in low light. These results suggest that DspA may influence gene expression in both a positive and a negative manner and that the dspA mutant behaves as if it were experiencing stress conditions (e.g., high-light exposure) even when maintained at near-optimal growth conditions for wild-type cells. This is discussed with respect to the importance of DspA for regulating the responses of the cell to environmental cues.

* Corresponding author. Mailing address: Department of Plant Biology, The Carnegie Institution of Washington, 260 Panama St., Stanford, CA 94305. Phone: (650) 325-1521, ext. 331. Fax: (650) 325-6857. E-mail: cjtu{at}.stanford.edu.

{dagger} This is manuscript no. 1632 from the Carnegie Institution.

Journal of Bacteriology, June 2004, p. 3889-3902, Vol. 186, No. 12
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.12.3889-3902.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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