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Journal of Bacteriology, July 2004, p. 4085-4099, Vol. 186, No. 13
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.13.4085-4099.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Microarray-Based Analysis of the Staphylococcus aureus {sigma}B Regulon

Markus Bischoff,1* Paul Dunman,2 Jan Kormanec,3 Daphne Macapagal,2 Ellen Murphy,4 William Mounts,5 Brigitte Berger-Bächi,1 and Steven Projan2

Department of Medical Microbiology, University of Zurich, CH-8028 Zurich, Switzerland,1 Infectious Diseases,2 Genomics, Wyeth Research, Pearl River, New York 10965,4 Genomics-Cambridge, Cambridge, Massachusetts 02140,5 Institute of Molecular Biology, Slovak Academy of Sciences, 84251 Bratislava, Slovak Republic3

Received 30 September 2003/ Accepted 18 March 2004

Microarray-based analysis of the transcriptional profiles of the genetically distinct Staphylococcus aureus strains COL, GP268, and Newman indicate that a total of 251 open reading frames (ORFs) are influenced by {sigma}B activity. While {sigma}B was found to positively control 198 genes by a factor of ≥2 in at least two of the three genetic lineages analyzed, 53 ORFs were repressed in the presence of {sigma}B. Gene products that were found to be influenced by {sigma}B are putatively involved in all manner of cellular processes, including cell envelope biosynthesis and turnover, intermediary metabolism, and signaling pathways. Most of the genes and/or operons identified as upregulated by {sigma}B were preceded by a nucleotide sequence that resembled the {sigma}B consensus promoter sequence of Bacillus subtilis. A conspicuous number of virulence-associated genes were identified as regulated by {sigma}B activity, with many adhesins upregulated and prominently represented in this group, while transcription of various exoproteins and toxins were repressed. The data presented here suggest that the {sigma}B of S. aureus controls a large regulon and is an important modulator of virulence gene expression that is likely to act conversely to RNAIII, the effector molecule of the agr locus. We propose that this alternative transcription factor may be of importance for the invading pathogen to fine-tune its virulence factor production in response to changing host environments.

* Corresponding author. Mailing address: Department of Medical Microbiology, University of Zurich, Gloriastr. 32, CH-8028 Zurich, Switzerland. Phone: 41 1 634 26 70. Fax: 41 1 634 49 06. E-mail: Bischoff{at}immv.unizh.ch.

Journal of Bacteriology, July 2004, p. 4085-4099, Vol. 186, No. 13
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.13.4085-4099.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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