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Journal of Bacteriology, July 2004, p. 4338-4349, Vol. 186, No. 13
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.13.4338-4349.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genomic DNA Microarray Analysis: Identification of New Genes Regulated by Light Color in the Cyanobacterium Fremyella diplosiphon

Emily L. Stowe-Evans,1 James Ford,1 and David M. Kehoe1,2*

Department of Biology,1 Center for Genomics and Bioinformatics, Indiana University, Bloomington, Indiana 474052

Received 6 December 2003/ Accepted 3 March 2004

Many cyanobacteria use complementary chromatic adaptation to efficiently utilize energy from both green and red regions of the light spectrum during photosynthesis. Although previous studies have shown that acclimation to changing light wavelengths involves many physiological responses, research to date has focused primarily on the expression and regulation of genes that encode proteins of the major photosynthetic light-harvesting antennae, the phycobilisomes. We have used two-dimensional gel electrophoresis and genomic DNA microarrays to expand our understanding of the physiology of acclimation to light color in the cyanobacterium Fremyella diplosiphon. We found that the levels of nearly 80 proteins are altered in cells growing in green versus red light and have cloned and positively identified 17 genes not previously known to be regulated by light color in any species. Among these are homologs of genes present in many bacteria that encode well-studied proteins lacking clearly defined functions, such as tspO, which encodes a tryptophan-rich sensory protein, and homologs of genes encoding proteins of clearly defined function in many species, such as nblA and chlL, encoding phycobilisome degradation and chlorophyll biosynthesis proteins, respectively. Our results suggest novel roles for several of these gene products and highly specialized, unique uses for others.

* Corresponding author. Mailing address: Department of Biology, Indiana University, Bloomington, IN 47405. Phone: (812) 856-4715. Fax: (812) 855-6705. E-mail: dkehoe{at}bio.indiana.edu.

Journal of Bacteriology, July 2004, p. 4338-4349, Vol. 186, No. 13
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.13.4338-4349.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shui, J., Saunders, E., Needleman, R., Nappi, M., Cooper, J., Hall, L., Kehoe, D., Stowe-Evans, E. (2009). Light-Dependent and Light-Independent Protochlorophyllide Oxidoreductases in the Chromatically Adapting Cyanobacterium Fremyella diplosiphon UTEX 481. Plant Cell Physiol 50: 1507-1521 [Abstract] [Full Text]  
  • Makarova, K. S., Koonin, E. V., Haselkorn, R., Galperin, M. Y. (2006). Cyanobacterial response regulator PatA contains a conserved N-terminal domain (PATAN) with an alpha-helical insertion. Bioinformatics 22: 1297-1301 [Abstract] [Full Text]  


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