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 Previous Article

Journal of Bacteriology, July 2004, p. 4417-4421, Vol. 186, No. 13
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.13.4417-4421.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Nitrosomonas europaea Expresses a Nitric Oxide Reductase during Nitrification

Hubertus J. E. Beaumont,* Bas van Schooten, Sylvia I. Lens, Hans V. Westerhoff, and Rob J. M. van Spanning

BioCentrum Amsterdam, Department of Molecular Cell Physiology, Vrije Universiteit, NL-1081 HV Amsterdam, The Netherlands

Received 20 February 2004/ Accepted 23 March 2004

In this paper, we report the identification of a norCBQD gene cluster that encodes a functional nitric oxide reductase (Nor) in Nitrosomonas europaea. Disruption of the norB gene resulted in a strongly diminished nitric oxide (NO) consumption by cells and membrane protein fractions, which was restored by the introduction of an intact norCBQD gene cluster in trans. NorB-deficient cells produced amounts of nitrous oxide (N2O) equal to that of wild-type cells. NorCB-dependent activity was present during aerobic growth and was not affected by the inactivation of the putative fnr gene. The findings demonstrate the presence of an alternative site of N2O production in N. europaea.

* Corresponding author. Present address: Evolutionary Genetics and Microbial Ecology Laboratory, School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand. Phone: 64 9 373 7599. Fax: 64 9 373 7416. E-mail: h.beaumont{at}auckland.ac.nz.

Journal of Bacteriology, July 2004, p. 4417-4421, Vol. 186, No. 13
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.13.4417-4421.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Cho, C. M.-H., Yan, T., Liu, X., Wu, L., Zhou, J., Stein, L. Y. (2006). Transcriptome of a Nitrosomonas europaea Mutant with a Disrupted Nitrite Reductase Gene (nirK).. Appl. Environ. Microbiol. 72: 4450-4454 [Abstract] [Full Text]  
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