This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Green, R. C.
Right arrow Articles by Darwin, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Green, R. C.
Right arrow Articles by Darwin, A. J.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 2004, p. 4910-4920, Vol. 186, No. 15
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.15.4910-4920.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

PspG, a New Member of the Yersinia enterocolitica Phage Shock Protein Regulon

Rebecca C. Green and Andrew J. Darwin*

Department of Microbiology, New York University School of Medicine, New York, New York 10016

Received 24 February 2004/ Accepted 23 April 2004

The Yersinia enterocolitica phage shock protein (Psp) system is induced when the Ysc type III secretion system is produced or when only the YscC secretin component is synthesized. Some psp null mutants have a growth defect when YscC is produced and a severe virulence defect in animals. The Y. enterocolitica psp locus is made up of two divergently transcribed cistrons, pspF and pspABCDycjXF. pspA operon expression is dependent on RpoN ({sigma}54) and the enhancer-binding protein PspF. Previous data indicated that PspF also controls at least one gene that is not part of the psp locus. In this study we describe the identification of pspG, a new member of the PspF regulon. Predicted RpoN-binding sites upstream of the pspA genes from different bacteria have a common divergence from the consensus sequence, which may be a signature of PspF-dependent promoters. The Y. enterocolitica pspG gene was identified because its promoter also has this signature. Like the pspA operon, pspG is positively regulated by PspF, negatively regulated by PspA, and induced in response to the production of secretins. Purified His6-PspF protein specifically interacts with the pspA and pspG control regions. A pspA operon deletion mutant has a growth defect when the YscC secretin is produced and a virulence defect in a mouse model of infection. These phenotypes were exacerbated by a pspG null mutation. Therefore, PspG is the missing component of the Y. enterocolitica Psp regulon that was previously predicted to exist.

* Corresponding author. Mailing address: Department of Microbiology, MSB 228, New York University School of Medicine, 550 First Ave., New York, NY 10016. Phone: (212) 263-3223. Fax: (212) 263-8276. E-mail: darwia01{at}med.nyu.edu.

Journal of Bacteriology, August 2004, p. 4910-4920, Vol. 186, No. 15
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.15.4910-4920.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Trotochaud, A. E., Wassarman, K. M. (2006). 6S RNA Regulation of pspF Transcription Leads to Altered Cell Survival at High pH. J. Bacteriol. 188: 3936-3943 [Abstract] [Full Text]  
  • Maxson, M. E., Darwin, A. J. (2006). Multiple promoters control expression of the Yersinia enterocolitica phage-shock-protein A (pspA) operon.. Microbiology 152: 1001-1010 [Abstract] [Full Text]  
  • Maxson, M. E., Darwin, A. J. (2005). Improved System for Construction and Analysis of Single-Copy {beta}-Galactosidase Operon Fusions in Yersinia enterocolitica. Appl. Environ. Microbiol. 71: 5614-5618 [Abstract] [Full Text]  
  • Venecia, K., Young, G. M. (2005). Environmental Regulation and Virulence Attributes of the Ysa Type III Secretion System of Yersinia enterocolitica Biovar 1B. Infect. Immun. 73: 5961-5977 [Abstract] [Full Text]  
  • Elderkin, S., Bordes, P., Jones, S., Rappas, M., Buck, M. (2005). Molecular Determinants for PspA-Mediated Repression of the AAA Transcriptional Activator PspF. J. Bacteriol. 187: 3238-3248 [Abstract] [Full Text]  
  • Lloyd, L. J., Jones, S. E., Jovanovic, G., Gyaneshwar, P., Rolfe, M. D., Thompson, A., Hinton, J. C., Buck, M. (2004). Identification of a New Member of the Phage Shock Protein Response in Escherichia coli, the Phage Shock Protein G (PspG). J. Biol. Chem. 279: 55707-55714 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»