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Journal of Bacteriology, August 2004, p. 5366-5375, Vol. 186, No. 16
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.16.5366-5375.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Role of the Pilot Protein YscW in the Biogenesis of the YscC Secretin in Yersinia enterocolitica

Peter Burghout,1 Frank Beckers,1 Emmie de Wit,1 Ria van Boxtel,1 Guy R. Cornelis,2 Jan Tommassen,1* and Margot Koster1

Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands,1 Division of Molecular Microbiology, Biozentrum, University of Basel, CH-4056 Basel, Switzerland2

Received 30 January 2004/ Accepted 6 May 2004

The YscC secretin is a major component of the type III protein secretion system of Yersinia enterocolitica and forms an oligomeric structure in the outer membrane. In a mutant lacking the outer membrane lipoprotein YscW, secretion is strongly reduced, and it has been proposed that YscW plays a role in the biogenesis of the secretin. To study the interaction between the secretin and this putative pilot protein, YscC and YscW were produced in trans in a Y. enterocolitica strain lacking all other components of the secretion machinery. YscW expression increased the yield of oligomeric YscC and was required for its outer membrane localization, confirming the function of YscW as a pilot protein. Whereas the pilot-binding site of other members of the secretin family has been identified in the C terminus, a truncated YscC derivative lacking the C-terminal 96 amino acid residues was functional and stabilized by YscW. Pulse-chase experiments revealed that ~30 min were required before YscC oligomerization was completed. In the absence of YscW, oligomerization was delayed and the yield of YscC oligomers was strongly reduced. An unlipidated form of the YscW protein was not functional, although it still interacted with the secretin and caused mislocalization of YscC even in the presence of wild-type YscW. Hence, YscW interacts with the unassembled YscC protein and facilitates efficient oligomerization, likely at the outer membrane.

* Corresponding author. Mailing address: Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands. Phone: (31) 30-2532999. Fax: (31) 30-2513655. E-mail: J.P.M.Tommassen{at}bio.uu.nl.

Journal of Bacteriology, August 2004, p. 5366-5375, Vol. 186, No. 16
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.16.5366-5375.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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