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Journal of Bacteriology, September 2004, p. 5775-5781, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5775-5781.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Assembly Dynamics of FtsZ Rings in Bacillus subtilis and Escherichia coli and Effects of FtsZ-Regulating Proteins

David E. Anderson,1 Frederico J. Gueiros-Filho,2,{dagger} and Harold P. Erickson1*

Department of Cell Biology, Duke University Medical Center, Durham, North Carolina,1 Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts2

Received 3 March 2004/ Accepted 26 May 2004

FtsZ is the major cytoskeletal component of the bacterial cell division machinery. It forms a ring-shaped structure (the Z ring) that constricts as the bacterium divides. Previous in vivo experiments with green fluorescent protein-labeled FtsZ and fluorescence recovery after photobleaching have shown that the Escherichia coli Z ring is extremely dynamic, continually remodeling itself with a half time of 30 s, similar to microtubules in the mitotic spindle. In the present work, under different experimental conditions, we have found that the half time for fluorescence recovery of E. coli Z rings is even shorter (~9 s). As before, the turnover appears to be coupled to GTP hydrolysis, since the mutant FtsZ84 protein, with reduced GTPase in vitro, showed an ~3-fold longer half time. We have also extended the studies to Bacillus subtilis and found that this species exhibits equally rapid dynamics of the Z ring (half time, ~8 s). Interestingly, null mutations of the FtsZ-regulating proteins ZapA, EzrA, and MinCD had only modest effects on the assembly dynamics. This suggests that these proteins do not directly regulate FtsZ subunit exchange in and out of polymers. In B. subtilis, only 30 to 35% of the FtsZ protein was in the Z ring, from which we conclude that a Z ring only 2 or 3 protofilaments thick can function for cell division.

* Corresponding author. Mailing address: Box 3709, Duke University Medical Center, Durham, NC 27710. Phone: (919) 684-6385. Fax: (919) 684-8090. E-mail: h.erickson{at}cellbio.duke.edu.

{dagger} Present address: Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, SP 05508-900, Brazil.

Journal of Bacteriology, September 2004, p. 5775-5781, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5775-5781.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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