This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barnett, T. C.
Right arrow Articles by Scott, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barnett, T. C.
Right arrow Articles by Scott, J. R.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 2004, p. 5865-5875, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5865-5875.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A Novel Sortase, SrtC2, from Streptococcus pyogenes Anchors a Surface Protein Containing a QVPTGV Motif to the Cell Wall

Timothy C. Barnett, Aman R. Patel, and June R. Scott*

Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia

Received 26 March 2004/ Accepted 27 May 2004

The important human pathogen Streptococcus pyogenes (group A streptococcus GAS), requires several surface proteins to interact with its human host. Many of these are covalently linked by a sortase enzyme to the cell wall via a C-terminal LPXTG motif. This motif is followed by a hydrophobic region and charged C terminus, which are thought to retard the protein in the cell membrane to facilitate recognition by the membrane-localized sortase. Previously, we identified two sortase enzymes in GAS. SrtA is found in all GAS strains and anchors most proteins containing LPXTG, while SrtB is present only in some strains and anchors a subset of LPXTG-containing proteins. We now report the presence of a third sortase in most strains of GAS, SrtC. We show that SrtC mediates attachment of a protein with a QVPTGV motif preceding a hydrophobic region and charged tail. We also demonstrate that the QVPTGV sequence is a substrate for anchoring of this protein by SrtC. Furthermore, replacing this motif with LPSTGE, found in the SrtA-anchored M protein of GAS, leads to SrtA-dependent secretion of the protein but does not lead to its anchoring by SrtA. We conclude that srtC encodes a novel sortase that anchors a protein containing a QVPTGV motif to the surface of GAS.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Emory University School of Medicine, Rollins Research Center, Atlanta, GA 30322. Phone: (404) 727-0402. Fax: (404) 727-8999. E-mail: scott{at}microbio.emory.edu.

Journal of Bacteriology, September 2004, p. 5865-5875, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5865-5875.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nakata, M., Koller, T., Moritz, K., Ribardo, D., Jonas, L., McIver, K. S., Sumitomo, T., Terao, Y., Kawabata, S., Podbielski, A., Kreikemeyer, B. (2009). Mode of Expression and Functional Characterization of FCT-3 Pilus Region-Encoded Proteins in Streptococcus pyogenes Serotype M49. Infect. Immun. 77: 32-44 [Abstract] [Full Text]  
  • Raz, A., Fischetti, V. A. (2008). Sortase A localizes to distinct foci on the Streptococcus pyogenes membrane. Proc. Natl. Acad. Sci. USA 105: 18549-18554 [Abstract] [Full Text]  
  • Fisher, M., Huang, Y.-S., Li, X., McIver, K. S., Toukoki, C., Eichenbaum, Z. (2008). Shr Is a Broad-Spectrum Surface Receptor That Contributes to Adherence and Virulence in Group A Streptococcus. Infect. Immun. 76: 5006-5015 [Abstract] [Full Text]  
  • LeMieux, J., Woody, S., Camilli, A. (2008). Roles of the Sortases of Streptococcus pneumoniae in Assembly of the RlrA Pilus. J. Bacteriol. 190: 6002-6013 [Abstract] [Full Text]  
  • Zahner, D., Scott, J. R. (2008). SipA Is Required for Pilus Formation in Streptococcus pyogenes Serotype M3. J. Bacteriol. 190: 527-535 [Abstract] [Full Text]  
  • Severin, A., Nickbarg, E., Wooters, J., Quazi, S. A., Matsuka, Y. V., Murphy, E., Moutsatsos, I. K., Zagursky, R. J., Olmsted, S. B. (2007). Proteomic Analysis and Identification of Streptococcus pyogenes Surface-Associated Proteins. J. Bacteriol. 189: 1514-1522 [Abstract] [Full Text]  
  • Barnett, T. C., Bugrysheva, J. V., Scott, J. R. (2007). Role of mRNA Stability in Growth Phase Regulation of Gene Expression in the Group A Streptococcus. J. Bacteriol. 189: 1866-1873 [Abstract] [Full Text]  
  • Lizano, S., Luo, F., Bessen, D. E. (2007). Role of Streptococcal T Antigens in Superficial Skin Infection. J. Bacteriol. 189: 1426-1434 [Abstract] [Full Text]  
  • Kratovac, Z., Manoharan, A., Luo, F., Lizano, S., Bessen, D. E. (2007). Population Genetics and Linkage Analysis of Loci within the FCT Region of Streptococcus pyogenes. J. Bacteriol. 189: 1299-1310 [Abstract] [Full Text]  
  • Podbielski, A. (2007). Flexible Architecture of the Streptococcus pyogenes FCT Genome Region: Finally the Clue for Understanding Purulent Skin Diseases and Long-Term Persistence?{triangledown}. J. Bacteriol. 189: 1181-1184 [Full Text]  
  • van Pijkeren, J.-P., Canchaya, C., Ryan, K. A., Li, Y., Claesson, M. J., Sheil, B., Steidler, L., O'Mahony, L., Fitzgerald, G. F., van Sinderen, D., O'Toole, P. W. (2006). Comparative and Functional Analysis of Sortase-Dependent Proteins in the Predicted Secretome of Lactobacillus salivarius UCC118.. Appl. Environ. Microbiol. 72: 4143-4153 [Abstract] [Full Text]  
  • LeMieux, J., Hava, D. L., Basset, A., Camilli, A. (2006). RrgA and RrgB Are Components of a Multisubunit Pilus Encoded by the Streptococcus pneumoniae rlrA Pathogenicity Islet. Infect. Immun. 74: 2453-2456 [Abstract] [Full Text]  
  • Marraffini, L. A., DeDent, A. C., Schneewind, O. (2006). Sortases and the Art of Anchoring Proteins to the Envelopes of Gram-Positive Bacteria. Microbiol. Mol. Biol. Rev. 70: 192-221 [Abstract] [Full Text]  
  • Davison, S., Couture-Tosi, E., Candela, T., Mock, M., Fouet, A. (2005). Identification of the Bacillus anthracis {gamma} Phage Receptor. J. Bacteriol. 187: 6742-6749 [Abstract] [Full Text]  
  • Kreikemeyer, B., Nakata, M., Oehmcke, S., Gschwendtner, C., Normann, J., Podbielski, A. (2005). Streptococcus pyogenes Collagen Type I-binding Cpa Surface Protein: EXPRESSION PROFILE, BINDING CHARACTERISTICS, BIOLOGICAL FUNCTIONS, AND POTENTIAL CLINICAL IMPACT. J. Biol. Chem. 280: 33228-33239 [Abstract] [Full Text]  
  • Boekhorst, J., de Been, M. W. H. J., Kleerebezem, M., Siezen, R. J. (2005). Genome-Wide Detection and Analysis of Cell Wall-Bound Proteins with LPxTG-Like Sorting Motifs. J. Bacteriol. 187: 4928-4934 [Abstract] [Full Text]  
  • Gaspar, A. H., Marraffini, L. A., Glass, E. M., DeBord, K. L., Ton-That, H., Schneewind, O. (2005). Bacillus anthracis Sortase A (SrtA) Anchors LPXTG Motif-Containing Surface Proteins to the Cell Wall Envelope. J. Bacteriol. 187: 4646-4655 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»