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Journal of Bacteriology, September 2004, p. 5945-5949, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5945-5949.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification of Operators and Promoters That Control SXT Conjugative Transfer

John W. Beaber and Matthew K. Waldor*

Department of Microbiology, Tufts University School of Medicine, and Howard Hughes Medical Institute, Boston, Massachusetts

Received 1 April 2004/ Accepted 24 May 2004

Transfer of SXT, a Vibrio cholerae-derived integrating conjugative element that encodes multiple antibiotic resistance genes, is repressed by SetR, a {lambda}434 cI-related repressor. Here we identify divergent promoters between s086 and setR that drive expression of the regulators of SXT transfer. One transcript encodes the activators of transfer, setC and setD. The second transcript codes for SetR and, like the cI transcript of lambda, is leaderless. SetR binds to four operators located between setR and s086; the locations and relative affinities of these sites suggest a model for regulation of SXT transfer.

* Corresponding author. Mailing address: Tufts University School of Medicine, 136 Harrison Ave., Jaharis 425, Boston, MA 02111. Phone: (617) 636-2730. Fax: (617) 636-2723. E-mail: matthew.waldor{at}tufts.edu.

Journal of Bacteriology, September 2004, p. 5945-5949, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5945-5949.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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