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Journal of Bacteriology, September 2004, p. 6298-6305, Vol. 186, No. 18
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.18.6298-6305.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Temporal Global Changes in Gene Expression during Temperature Transition in Yersinia pestis

Vladimir L. Motin,1,{dagger} Anca M. Georgescu,1 Joseph P. Fitch,1 Pauline P. Gu,1 David O. Nelson,1 Shalini L. Mabery,1 Janine B. Garnham,1 Bahrad A. Sokhansanj,1 Linda L. Ott,1 Matthew A. Coleman,1 Jeffrey M. Elliott,1 Laura M. Kegelmeyer,1 Andrew J. Wyrobek,1 Thomas R. Slezak,1 Robert R. Brubaker,2 and Emilio Garcia1*

Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, California,1 Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan2

Received 8 August 2003/ Accepted 8 June 2004

DNA microarrays encompassing the entire genome of Yersinia pestis were used to characterize global regulatory changes during steady-state vegetative growth occurring after shift from 26 to 37°C in the presence and absence of Ca2+. Transcriptional profiles revealed that 51, 4, and 13 respective genes and open reading frames (ORFs) on pCD, pPCP, and pMT were thermoinduced and that the majority of these genes carried by pCD were downregulated by Ca2+. In contrast, Ca2+ had little effect on chromosomal genes and ORFs, of which 235 were thermally upregulated and 274 were thermally downregulated. The primary consequence of these regulatory events is profligate catabolism of numerous metabolites available in the mammalian host.

* Corresponding author. Mailing address: Biology and Biotechnology Research Program, L-452, 7000 East Ave., Livermore, CA 94550. Phone: (925) 422-8002. Fax: (925) 422-2282. E-mail: garcia12{at}llnl.gov.

{dagger} Present address: Departments of Pathology and Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555.

Journal of Bacteriology, September 2004, p. 6298-6305, Vol. 186, No. 18
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.18.6298-6305.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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