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Journal of Bacteriology, September 2004, p. 6325-6326, Vol. 186, No. 18
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.18.6325-6326.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Recombinant Cyclophilins Lack Nuclease Activity

Angel Manteca and Jesus Sanchez*

Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Oviedo, Spain

Received 7 April 2004/ Accepted 9 June 2004

Several single-domain prokaryotic and eukaryotic cyclophilins have been identified as also being unspecific nucleases with a role in DNA degradation during the lytic processes that accompany bacterial cell death and eukaryotic apoptosis. Evidence is provided here that the supposed nuclease activity of human and bacterial recombinant cyclophilins is due to contamination of the proteins by the host Escherichia coli endonuclease and is not an intrinsic property of these proteins.

* Corresponding author. Mailing address: Universidad de Oviedo, Departamento de Biología Funcional, Area de Microbiologia, Julian Claveria s/n, Oviedo 33006, Spain. Phone: 34 985103555. Fax: 34 985103148. E-mail: jsm{at}fq.uniovi.es.

Journal of Bacteriology, September 2004, p. 6325-6326, Vol. 186, No. 18
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.18.6325-6326.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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