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Journal of Bacteriology, October 2004, p. 6409-6421, Vol. 186, No. 19
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.19.6409-6421.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of Three New Competence-Regulated Operons in Haemophilus influenzae

Timothy M. VanWagoner,1,2 Paul W. Whitby,1 Daniel J. Morton,1 Thomas W. Seale,1 and Terrence L. Stull1,3*

Departments of Pediatrics,1 Microbiology/Immunology, University of Oklahoma Health Sciences Center, Oklahoma City,3 Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma2

Received 17 November 2003/ Accepted 29 June 2004

Haemophilus influenzae is one of a growing number of bacteria in which the natural ability to uptake exogenous DNA for potential genomic transformation has been recognized. To date, several operons involved in transformation in this organism have been described. These operons are characterized by a conserved 22-bp regulatory element upstream of the first gene and are induced coincident with transfer from rich to nutrient-depleted media. The previously identified operons comprised genes encoding proteins that include members of the type II secretion system and type IV pili, shown to be essential for transformation in other bacteria, and other proteins previously identified as required for transformation in H. influenzae. In the present study, three novel competence operons were identified by comparative genomics and transcriptional analysis. These operons have been further characterized by construction of null mutants and examination of the resulting transformation phenotypes. The putative protein encoded by the HI0366 gene was shown to be essential for DNA uptake, but not binding, and is homologous to a protein shown to be required for pilus biogenesis and twitching motility in Pseudomonas aeruginosa. An insertion in HI0939 abolished both DNA binding and uptake. The predicted product of this gene shares characteristics with PulJ, a pseudopilin involved in pullulanase export in Klebsiella oxytoca.

* Corresponding author. Mailing address: Department of Pediatrics, CHO 2308, 940 NE 13th St., Oklahoma City, OK 73104. Phone: (405) 271-4401. Fax: (405) 271-8710. E-mail: terrence-stull{at}ouhsc.edu.

Journal of Bacteriology, October 2004, p. 6409-6421, Vol. 186, No. 19
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.19.6409-6421.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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