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Journal of Bacteriology, October 2004, p. 6661-6666, Vol. 186, No. 19
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.19.6661-6666.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Major Role of NAD-Dependent Lactate Dehydrogenases in Aerobic Lactate Utilization in Lactobacillus plantarum during Early Stationary Phase

Philippe Goffin,1 Frédérique Lorquet,1,{dagger} Michiel Kleerebezem,2 and Pascal Hols1*

Unité de Génétique, Institut des Sciences de la Vie, Université Catholique de Louvain, Louvain-la-Neuve, Belgium,1 Wageningen Centre for Food Sciences, NIZO Food Research, Ede, The Netherlands2

Received 5 April 2004/ Accepted 9 July 2004

NAD-independent lactate dehydrogenases are commonly thought to be responsible for lactate utilization during the stationary phase of aerobic growth in Lactobacillus plantarum. To substantiate this view, we constructed single and double knockout mutants for the corresponding genes, loxD and loxL. Lactate-to-acetate conversion was not impaired in these strains, while it was completely blocked in mutants deficient in NAD-dependent lactate dehydrogenase activities, encoded by the ldhD and ldhL genes. We conclude that NAD-dependent but not NAD-independent lactate dehydrogenases are involved in this process.

* Corresponding author. Mailing address: Unité de Génétique, Université Catholique de Louvain, Place Croix du Sud 5, B-1348 Louvain-la-Neuve, Belgium. Phone: 32-10-478896. Fax: 32-10-473109. E-mail: hols{at}gene.ucl.ac.be.

{dagger} Present address: Génétique Microbienne, INRA, 78352 Jouy en Josas cedex, France.

Journal of Bacteriology, October 2004, p. 6661-6666, Vol. 186, No. 19
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.19.6661-6666.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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